The Nairobi Expressway toll stations from the JKIA to Westlands highlight key points along the road.
The stations will be operating as a means of ticketing through which payments will be done by the road users.
Motorists will be charged differently depending on their destinations.
Last week, the government gazetted price charges for using the Nairobi Expressway once the project is open for public use.
According to the Gazette notice, each driver on the Nairobi Expressway will be required to pay a fee of between Sh310 and Sh100 to use the new road. The charges will depend on the distance travelled.
The gazette notice also indicated what will not be allowed to use the expressway.
Two-wheeled motor vehicles were barred from using the expressway.
Transport CS James Macharia announced that the drivers will pay Sh310 from Mlolongo to James Gichuru and Sh260 from Jame Gichuru to JKIA and vice versa.
The Sh72 billion project is a public-private partnership funded, with no debt incurred by the government.
China Communications Construction Company, the parent firm of China Road and Bridge Corporation, is funding and building the highway.
The expressway will cut travel time from Rironi to JKIA from three hours to just 20 minutes.